Plants / Gene / Transgenic / Genes / Protein / Biomass

PIIN 05330 transgenic Arabidopsis plants enhanced drought-stress tolerance

Posted On: Mar 21, 2023   |   Posted By: Springer

Abstract
Serendipita indica is a root endophytic fungus and can symbiose with many plant species. Effector proteins secreted by fungi show many functions during symbiosis or infection. To investigate the role of the protein PIIN 05330 secreted by S. indica , the gene PIIN 05330 was cloned and its transgenic Arabidopsis lines were constructed. The transgenic lines were evaluated for their drought tolerance. The results showed that these transgenic Arabidopsis lines were sensitive to drought stress during the germination stage. Compared with Arabidopsis wild type and the empty vector lines seedlings, the transgenic lines had higher total root surface area, root diameters, total root volumes, relative root elongation rates, total root length, and root tip numbers. In addition, the transgenic lines showed higher aboveground biomass and chlorophyll levels. Compared with WT and VT lines, activities of SOD, POD and CAT increased in the transgenic Arabidopsis lines under drought condition, especially their...

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