Human papillomavirus integration signature in cervical lesions: identification of MACROD2 gene as HPV hot spot integration site
Abstract
Background
High-risk HPV is clearly associated with cervical cancer. Integration of HPV DNA into the host genome is considered a key event in driving cervical carcinogenesis. However, the mechanism on how HR-HPV integration influences the host genome structure has remained enigmatic.
Methods
In our study, 25 DNA samples including 11 from fresh-frozen cervical carcinomas and 14 from fresh-frozen high-grade squamous intraepithelial lesion were detected using the method of HPV capture combined with next generation sequencing.
Results
We calculated the frequency in each viral gene or region and found that breakpoints were prone to occur in L1 and L2 instead of E2 in the cervical cancer ( P = 0.0004 and P = 5.15 × 10 −40 ) and HSIL group ( P = 2.1 × 10 −32 and P = 7.06 × 10 −13 ). The results revealed that HPV16 showed a strong tendency toward intronic region but a subtle tendency toward intergenic region. The most frequent integration site was in the...
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Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...
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