Genes / Immunity / Gene / Biosynthesis

Biosynthesis and transfer of 2-acetamido-4-methylamino-l-fucose to the core-lipid A in Bordetella pertussis

Posted On: Mar 21, 2023   |   Posted By: Springer

Abstract
The trisaccharide unit in lipopolysaccharide of Bordetella pertussis is important for immune recognition, but how the rare sugar 2-acetamido-4-methylamino-fucose in the trisaccharide unit is synthesized and transferred to the core of lipopolysaccharide in B. pertussis remains unclear. In this study, we demonstrated that the genes bplF , bplG , bplL and bplI are involved in the synthesis and transfer of 2-acetamido-4-methylamino-fucose to the core of lipopolysaccharide in B. pertussis. These genes were overexpressed with various combination in a recombinant E. coli strain which can synthesize B. pertussis core-lipid A. Lipopolysaccharides were isolated from these strains and analyzed by using SDS-PAGE, ion chromatography and NMR. Based on these analyses, the mechanism of biosynthesis and transfer of 2-acetamido-4-methylamino-l-fucose to the core-lipid A in B. pertussis is proposed.

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