Virus

Quality Control Testing, Characterization and Critical Quality Attributes of Adeno‐Associated Virus Vectors Used for Human Gene Therapy

15 days ago   |   By Wiley

This article describes quality control testing and characterization of adeno‐associated virus gene therapy vectors for clinical product development. It explores the relationship between AAV vector design and manufacturing process design with product critical quality attributes required for safety and durable efficacious expression, with emphasis on vector immunogenicity.
Abstract
For adeno‐associated virus ‐based human gene therapy, challenges for the translation of promising research results to successful clinical development include optimization of vector design and manufacturing...
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Evaluation of the Single‐Use Fixed‐Bed Bioreactors in Scalable Virus Production

Evaluation of the Single‐Use Fixed‐Bed Bioreactors in Scalable Virus Production

15 days ago   |   By Wiley

Adherent cells can attach and grow in three‐dimensional fixed‐bed matrix. The single use disposable upstream technology has been implemented in many different viral vector and vaccine productions. Main process steps in viral production contain cell expansion phase, induction of the virus production and harvest.
Abstract
The accelerating development of gene therapy from research towards clinical trials and beyond has elevated the demand for practical viral vector‐manufacturing solutions. The use of disposable upstream technology is gaining traction in clinical manufacturing. Packed‐bed or...
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Outside Front Cover:

Outside Front Cover:

15 days ago   |   By Wiley

Stable cell lines provide scalable and high yielding production systems enabling the development of efficient manufacturing bioprocesses, essential for the supply of viral vectors for gene and cell therapies. The establishment of lentiviral vector producer cell lines is however very challenging. In this review, the major bottlenecks and achievements in the development of lentiviral vector stable cell lines are discussed. This is reported by Mariana V. Ferreira, Elisa T. Cabral, Ana Sofia Coroadinha in the article 2000017 .
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Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production

Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production

15 days ago   |   By Wiley

The baculovirus/insect cell is a standard platform for manufacturing of adeno‐associated viral vectors. This study compares homologous recombination and transposition to generate the baculovirus expressing vectors. Both methods allow generation of high titers of AAV vectors, but BEVs generated by HR are genetically more stable and devoid of any bacterial DNA sequences.
Abstract
Viral vectors have a great potential for gene delivery, but manufacturing is a big challenge for the industry. The baculovirus‐insect cell is one of the most scalable platforms to produce recombinant...
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Separating Empty and Full Recombinant Adeno‐Associated Virus Particles Using Isocratic Anion Exchange Chromatography

Separating Empty and Full Recombinant Adeno‐Associated Virus Particles Using Isocratic Anion Exchange Chromatography

15 days ago   |   By Wiley

Robust and scalable separation of empty particles from recombinant adeno‐associated virus vectors is key in rAAV production. This separation is currently achieved via linear gradient elution chromatography which is challenging to implement at large scale. Improved separation resolution is demonstrated between the empty and full particles by combining NaCl and MgCl 2 as eluents, enabling an isocratic anion exchange chromatography separation.
Abstract
The development of recombinant adeno‐associated virus gene therapies is becoming an increasing priority in the biotherapeutic landscape. One...
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Production of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparison of Platform Technologies for Viral Vector Production

Production of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparison of Platform Technologies for Viral Vector Production

15 days ago   |   By Wiley

Modified Vaccinia Ankara virus is a viral vector used for gene therapies. Here, MVA virus is produced with AGE1.CR.pIX suspension cells using different platform technologies. The authors then compared and discussed the pros and cons between the different platform technologies, based on the virus production yields and other criteria such as scalability and process robustness.
Abstract
Modified Vaccinia Ankara virus is a promising vector for vaccination against various challenging pathogens or the treatment of some types of cancers, requiring a high amount of virions per dose for vaccination...
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World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

2nd International Conference on Tissue Science and Regenerative Medicine

This conference is designed for experts in academia and industries working in the tissue science and regenerative field, this conference will examine cutting-edge research in... Read more ...

Bond LSC Researchers Named Most Highly Cited for 2020

Bond LSC Researchers Named Most Highly Cited for 2020

Bond Life Sciences Center principal investigators Bing Yang and Ron Mittler are pictured above. | photos by Erica Overfelt, Bond LSC. By Lauren Hines | Bond LSC Building onto... Read more ...

A New Way to Measure Record-Setting Electron Beams

A New Way to Measure Record-Setting Electron Beams

Sam Barber, left, a research scientist at Berkeley Lab's BELLA Center, and Jeroen van Tilborg, a staff scientist at the BELLA Center, hold the active plasma lens, right, and... Read more ...

Researchers Find Cerebrospinal Fluid Boosts Cancer Cell Aggression

Researchers Find Cerebrospinal Fluid Boosts Cancer Cell Aggression

In cell studies, Mayo researchers and collaborators have uncovered why tumor cells from glioblastoma are more aggressive in one part of the brain than in others. In an article... Read more ...

Historical evolution of spheroids and organoids, and possibilities of use in...

Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

Abstract Background An impressive percentage of biomedical advances were achieved through animal research and cell culture investigations. For drug testing and disease... Read more ...

PSC Software™ Announces the Release of Microsoft Office 365 Integration in...

PSC Software™ Announces the Release of Microsoft Office 365 Integration in Adaptive Compliance Engine® Version 3.5.0

Written By: PSC Software Business Development and Marketing Teams PSC Software , a wholly owned subsidiary of PSC Biotech Corporation , is pleased to announce the integration... Read more ...

The Importance of Good Documentation Practices

The Importance of Good Documentation Practices

Written By: Akshaya Mohan, Regulatory Affairs Manager, PSC Biotech® Documentation is a “record” or “evidence” of an event that has happened. In the life-science industry “If it... Read more ...

Genome-editing tool TALEN outperforms CRISPR-Cas9 in tightly packed DNA

Researchers used single-molecule imaging to compare the genome-editing tools CRISPR-Cas9 and TALEN. Their experiments revealed that TALEN is up to five times more efficient... Read more ...

Blocking drug efflux mechanisms facilitate genome engineering process in...

Penicillium funiculosum NCIM1228 is a non-model filamentous fungus that produces high-quality secretome for lignocellulosic biomass saccharification. Despite having desirable... Read more ...

Approaches to genetic tool development for rapid domestication of non-model...

Non-model microorganisms often possess complex phenotypes that could be important for the future of biofuel and chemical production. They have received significant interest the... Read more ...

Design Therapeutics Secures $125 Million in Series B Financing to Advance...

SAN DIEGO--Design Therapeutics Secures $125 Million in Series B Financing to Advance Pipeline of Genomic Medicines for Nucleotide Repeat Expansion Disorders... Read more ...